fvb n mice  (Jackson Laboratory)

Journal: Cell & Bioscience

Article Title: Overexpression of ONECUT1 suppresses hepatoblastoma progression via modulating tumor cell growth and tumor microenvironment

doi: 10.1186/s13578-026-01569-0

Figure Lengend Snippet: Overexpression of ONECUT1 inhibits YAPS127A/ΔN90-β-catenin driven hepatocarcinogenesis in FVB/N mice. A Schematic representation of the plasmid constructs used in the study: EF1α-driven ΔN90-β-catenin and YAPS127A, TRE-ONECUT1 with rTTA, and SB transposase. B Cartoon diagram of the hydrodynamic tail-vein injection (HTVI) procedure used to deliver the plasmid mixture into FVB/N mouse hepatocytes and experimental timeline: mice were allowed to develop palpable liver tumors before switching to DOX-containing drinking water to induce ONECUT1 expression. C Western blot showing protein expression in liver tumors under indicated conditions. ONECUT1 is robustly induced after DOX treatment. Myc-tag indicates ΔN90-β-catenin expression, and Cyclin D1 expression is markedly decreased following ONECUT1 overexpression. GAPDH serves as loading control. D IHC staining for ONECUT1 in mice subjected to pretreatment and ONECUT1 overexpression for 1, 2, and 3 days. Scale bar: 50 μm (200×). E Liver weights. F Liver-to-body weight ratio. G Survival of YAPS127A/ΔN90-β-catenin mice with or without DOX. H Representative gross images of tumor-bearing livers before treatment, DOX−, and at indicated time points after DOX induction (1 day, 3 days, 7 days, and 8 weeks). Liver weight is shown in the upper left corner of the image. Statistical significance was determined by a two-tailed unpaired Student’s t-test. Data are presented as mean ± SD. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: For this study, wild-type FVB/N mice were obtained from the Jackson Laboratory (Sacramento, CA).

Techniques: Over Expression, Plasmid Preparation, Construct, Injection, Expressing, Western Blot, Control, Immunohistochemistry, Two Tailed Test

Journal: Cell & Bioscience

Article Title: Overexpression of ONECUT1 suppresses hepatoblastoma progression via modulating tumor cell growth and tumor microenvironment

doi: 10.1186/s13578-026-01569-0

Figure Lengend Snippet: Depletion of T cells partially inhibits ONECUT1-induced hepatoblastoma regression in YAPS127A/ΔN90-β-catenin mice. A Experimental design. FVB/N mice were hydrodynamically injected with YAPS127A/ΔN90-β-catenin/TRE-ONECUT1 plasmids on Day − 24. On Day − 5, mice received i.p. injections of control IgG or anti-CD4 plus anti-CD8 antibodies (CD4/CD8-Ab). On Day 0, DOX treatment was initiated to induce ONECUT1 expression or regular water as a DOX (−) control, accompanied by the same antibody regimen. Mice were sacrificed on Day 3 after DOX induction or the corresponding time point in DOX (−) groups. Four experimental groups were included: DOX (+) + IgG, DOX (+) + CD4/CD8-Ab, DOX (−) + IgG, and DOX (−) + CD4/CD8-Ab. B , C Quantification of liver weight ( B ) and liver-to-body weight ratio ( C ). Under DOX (+) conditions, T-cell depletion significantly attenuated ONECUT1-induced tumor regression (* P < 0.05). In contrast, under DOX (−) conditions, CD4/CD8 depletion did not significantly affect liver weight or liver-to-body weight ratio. Statistical significance was determined by a two-tailed unpaired Student’s t-test. Data are presented as mean ± SD. ns, not significant; * P < 0.05; *** P < 0.001; **** P < 0.0001. D Representative gross liver morphology and histological analyses. Images from DOX (+) groups are shown. H&E staining (40×) shows larger tumor burden in CD4/CD8-depleted mice. Ki67 immunostaining (200×) indicates higher proliferation in CD4/CD8-Ab–treated livers. CD4 and CD8 immunostaining (200×) confirms successful T-cell depletion.

Article Snippet: For this study, wild-type FVB/N mice were obtained from the Jackson Laboratory (Sacramento, CA).

Techniques: Injection, Control, Expressing, Two Tailed Test, Staining, Immunostaining